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Image Search Results
Journal: PLoS ONE
Article Title: Derivation and Expansion Using Only Small Molecules of Human Neural Progenitors for Neurodegenerative Disease Modeling
doi: 10.1371/journal.pone.0059252
Figure Lengend Snippet: (A) Plated EBs differentiating in the presence of both PMA and CHIR for 6 days. (B) Phase-contrast images of neural epithelial cells on the indicated days after splitting. (C) Immunostaining of hESC-derived neural epithelial cells with antibodies raised against the indicated neural progenitor markers. Nuclei are counterstained with Hoechst. (D) Immunostaining of spontaneously differentiated neural epithelial cells for TUBBIII and NEUN, for GFAP and S100-beta after astrocyte differentiation, as well as O4 and OLIG2 after spontaneous differentiation, indicating oligodendrocyte formation. Scale bars are 100 µm. See also .
Article Snippet: The primary antibodies used in this study are mouse anti-NESTIN (1∶150, R&D), goat anti-SOX1 (1∶150, R&D), rabbit anti-PAX6 (1∶300, Covance), goat anti-SOX2 (1∶200, Santa Cruz), mouse anti-FOXA2 (1∶100, Santa Cruz), rabbit anti-TH (1∶500, Pel Freez), sheep anti-TH (1∶400, Pel Freez), mouse anti-TUBBIII (1∶1000, Covance), rabbit anti-TUBBIII (1∶2000, Covance), rabbit anti-MAP2 (1∶1000, Santa Cruz), mouse anti-O4 (1∶100, Millipore), rabbit anti-ISLET1 (1∶1500, Abcam), mouse anti-HB9 (5 µg/mL own preparation from DSHB hybridoma), goat anti-CHAT (1∶100, Millipore), mouse anti-BRN3A (1∶500, Santa Cruz), rabbit anti-PERIPHERIN (1∶200, Millipore), rabbit anti-GFAP (1∶1000, Dako), mouse anti GFAP (1∶500, Millipore), mouse anti
Techniques: Immunostaining, Derivative Assay
Journal: PLoS ONE
Article Title: Derivation and Expansion Using Only Small Molecules of Human Neural Progenitors for Neurodegenerative Disease Modeling
doi: 10.1371/journal.pone.0059252
Figure Lengend Snippet: Summary of the markers used in this study as well as the characteristics of NSCs, lt-hESNSCS, R-NCs, pNSCs, and smNPCs.
Article Snippet: The primary antibodies used in this study are mouse anti-NESTIN (1∶150, R&D), goat anti-SOX1 (1∶150, R&D), rabbit anti-PAX6 (1∶300, Covance), goat anti-SOX2 (1∶200, Santa Cruz), mouse anti-FOXA2 (1∶100, Santa Cruz), rabbit anti-TH (1∶500, Pel Freez), sheep anti-TH (1∶400, Pel Freez), mouse anti-TUBBIII (1∶1000, Covance), rabbit anti-TUBBIII (1∶2000, Covance), rabbit anti-MAP2 (1∶1000, Santa Cruz), mouse anti-O4 (1∶100, Millipore), rabbit anti-ISLET1 (1∶1500, Abcam), mouse anti-HB9 (5 µg/mL own preparation from DSHB hybridoma), goat anti-CHAT (1∶100, Millipore), mouse anti-BRN3A (1∶500, Santa Cruz), rabbit anti-PERIPHERIN (1∶200, Millipore), rabbit anti-GFAP (1∶1000, Dako), mouse anti GFAP (1∶500, Millipore), mouse anti
Techniques: